DEsigning Primers

Bradley Turner bsturner at mbcrr.harvard.edu
Wed Jun 13 17:51:52 EST 2001


Hello Joseph,

You can try one or some of the following sites.
The first site has an annotated/comparative 
list of primer design sites.
[no affiliation with any of the sites, some of which
are commercial in origin] 

http://www.alkami.com/primers/refdsgn.htm
http://eatworms.swmed.edu/~tim/primerfinder/
http://ww-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi
http://www.embl-heidelberg.de/~toldo/JaMBW/5/2/

You can analyze your primers at:

http://207.32.43.248/oligocalc.asp

Hope this helps,
Brad Turner

****************************************************************
                    Bradley Turner
                Beth Israel Deaconess
                    Medical Center

Harvard Medical School          617-667-1215 phone
Division of Gastroenterology    617-667-2767 fax
Room Dana 605                   bsturner at biosun.harvard.edu
330 Brookline Avenue            bturner at caregroup.harvard.edu
Boston, MA 02215                bsturner at mbcrr.harvard.edu
****************************************************************
                   Graduate Student 
      Molecular Biology, Cell Biology, Biochemistry
                  Boston University
                   bsturner at bu.edu
****************************************************************


On 13 Jun 2001, Joseph wrote:

> Does anyone know a good website with software that will help you design
> primers for DNA sequencing.
> 
> 
> <http://www.biowww.net/forum/read.php?f=6&i=460&t=460>
> 
> 

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