E. coli preservation

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Thu Jun 14 08:35:34 EST 2001


In <001701c0f4b3$34b0cfe0$8c01a8c0 at tn.nic.in>, R. Jayakumar wrote:

>
>----- Original Message -----
>From: "Nick Theodorakis" <nicholas_theodorakis at urmc.rochester.edu>
>To: <methods at hgmp.mrc.ac.uk>
>Sent: Wednesday, June 13, 2001 11:17 PM
>Subject: Re: E. coli preservation
>
>
>> In <00c401c0f42a$7238c220$8c01a8c0 at tn.nic.in>, R. Jayakumar 
wrote:
>> >
>> >Stab culture -- Mostly everybody would have thought of it,, but the
>> >gentleman wanted to know something that would preserve his cells 
for
>> years
>> >and not a few weeks which stab cultures do.
>>
>> I used to routinely gets live bugs form 2-3  year old stab cultures.
>>
>> When I was a grad student, that was our labs preferred method of bug
>> storage.
>>
>

>
>the more important question here is did you store them in room 
temperature.
>If so, then your experience will be useful to me too..
>  thanks in advance

Yes. Room temperature, in the dark (a drawer). With a glass vial that you 
can close very tightly to keep the air out.

Nick


-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu

 




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