E. coli preservation

R. Jayakumar jakku at mrna.tn.nic.in
Thu Jun 14 10:04:54 EST 2001


Did you try sealing the cap with parafilm.  Does it still dry?
  regards
jayakumar


----- Original Message -----
From: "Dr. Duncan Clark" <news at hgmp.mrc.ac.uk>
To: <methods at hgmp.mrc.ac.uk>
Sent: Thursday, June 14, 2001 7:57 PM
Subject: Re: E. coli preservation


> In article <v04011701b74e6d5f5719@[144.92.64.179]>, the eminent Michael
> L. Sullivan at BIOSCI/MRC Human Genome Mapping Project Resource Centre
> wrote
> >So I suppose I am old enough (38?) to at least have had the knowleged
> >passed down to me by an elder!  I was waiting for somebody who actually
had
> >experience doing it to post.  I'm sort of amazed you are the only one to
> >suggest it!
>
> The problem with stabs is sealing them praperly. I used to use 5ml glass
> bijous and then candle wax sealed the metal cap. I still have some from
> maybe 15 years ago though whether they are alive I do not know. Stabs
> done in screw capped eppendorfs also work but tend to dry over 3 -
> 5years although I'm pretty sure somewhere in a cupboard I have some from
> maybe 10 years ago. With both of these you can autoclave the agar in the
> tube. Not much good for plates though :-(
>
> Duncan
> --
> It might look like I'm doing nothing, but at the cellular level I'm really
> quite busy.
>
> Dr. Duncan Clark
> GeneSys Ltd.
> Tel. +44 (0) 1252376288
> FAX +44 (0) 8701640382
>
>

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