rna from woody plant tissues

David J. Meyer meyerdj at phibred.com
Fri Jun 15 15:27:40 EST 2001


I think that you are correct in your evaluation of the amount of tissue
which will be needed from this source.
--
David J. Meyer, Ph.D.
Pioneer Hi-Bred International, Inc.
A DuPont Company
7300 NW 62nd Ave
PO Box 1004
Johnston, IA   50131-1004

""Michael L. Sullivan"" <mlsulliv at facstaff.wisc.edu> wrote in message
news:v04011701b74410097b48@[144.92.64.179]...
> Does anybody have reccomendations for preparing RNA from woody plant
> tissues.  I'd be especially interested in anyone who's successfully
> modified a triazol protocol.  I've been able to get RNA, but at quite low
> yields (e.g. 30 ug per gram of tissue).
>
> One thought is that I just really need to use a lot of tissue, because
that
> actual amount of "cell contents" is relatively small compared to the
amount
> of "junk".
>
> Thanks for any input.
>
> Mike
>
> Michael L. Sullivan, Ph.D
>
> U.S. Dairy Forage Research Center
> 1925 Linden Drive West
> Madison WI, 53706
>
> (608) 264-5144 Phone
> (608) 264-5147 Fax
>
> ---





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