cloning of PCR products - errors?
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Mon Jun 18 06:16:12 EST 2001
In article <jp9d782uk05.fsf at sirppi.helsinki.fi>,
Kristo K M Kulju <kulju at sirppi.helsinki.fi> wrote:
> There is lot of publications about PCR
> errors but what about cloning procedure? Is is considered to be
> practically "error free"?
Yes. That's why it's called 'cloning'.
You could get errors at the ligation sites if you were dead unlucky (but
it probably wouldn't clone anyway) and any bug that manages to introduce
errors into a 3 kb plasmid during replication has serious problems . . .
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
Twinkle, twinkle little star, I don't wonder what you are;
Because of spectroscopic gen, I know you're made of hydrogen.
Twinkle, twinkle little star, Now I know just what you are.
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