DNA storage

Frank O. Fackelmayer Frank at Fackelmayer.de
Mon Jun 18 06:26:37 EST 2001



"J. Erdmann" wrote:
> 
> Hello,
> what is your opinion about optimal DNA storage condition and why?
> thanks in advance
> 

usually CLEAN DNA is stable in TE at 4°C. However, we use the following conditions:

clean plasmid DNA, long term storage: frozen in TE (-20°C)
clean plasmid DNA, short term: in TE at 4°C
crude miniprep plasmid DNA: frozen in TE (-20°C), also for short term storage.

long DNA (genomic DNA, lambda DNA): in TE at 4°C (to avoid shearing
forces during freezing/thawing)




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