Best plant RNA kit?

Allison MacKay amackay at uoguelph.ca
Mon Jun 18 15:20:03 EST 2001


I was wondering if I could get a few suggestions about which
RNA extraction kit to use for plant tissue?  My species is a
small, non-woody weed (sort of like Arabidopsis in consistency
actually!).  I've been using Ambion's RNAqueous-midi kit (with
homogenization by grinding in liquid N2 followed by Dounce) but
yields have been downright pathetic (<100 ug per g of tissue)!
I've heard that RNEasy from Qiagen is good.  Any advice would be
greatly appreciated.

--
Allison Mackay
Plant Cell Technology Laboratory
Department of Plant Agriculture
University of Guelph




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