E. coli preservation

Rafael Maldonado rmaldonado at ua.es
Tue Jun 19 05:12:24 EST 2001



Chris Fields wrote:
> 
> My professor has several strains of E. coli and Salmonella in stab
> cultures that are still viable after 20 years!  I think that you have to
> be VERY careful with the phenotype.  A few of his were DOA due to
> thymineless death (had a thyA mutation).  I guess his grad student at
> the time had left out the thymidine; my prof was a bit pissed.

PLasmids can be lost vith variable frequency in stab, even F primes...

-- 
Rafael Maldonado, Ph.D.
Division of Genetics
University of Alicante (Spain)




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