E. coli preservation
rmaldonado at ua.es
Tue Jun 19 05:12:24 EST 2001
Chris Fields wrote:
> My professor has several strains of E. coli and Salmonella in stab
> cultures that are still viable after 20 years! I think that you have to
> be VERY careful with the phenotype. A few of his were DOA due to
> thymineless death (had a thyA mutation). I guess his grad student at
> the time had left out the thymidine; my prof was a bit pissed.
PLasmids can be lost vith variable frequency in stab, even F primes...
Rafael Maldonado, Ph.D.
Division of Genetics
University of Alicante (Spain)
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