E. coli preservation

Tim Spahlinger txs at po.cwru.edu
Tue Jun 19 13:28:11 EST 2001

Not to belabor the point, but I just happen to have found
Sambrook's and Russell's procedures for Storage of Bacterial
Cultures in "Molecular Cloning", Third Edition, page A8.5
(appendix 8).  (I also needed to do some storage, so I
looked them up!).  They don't give a relative time limit for
the storage of stab cultures.

By their methods, a final concentration (v/v) of 15%
glycerol seems sufficient for long term storage of the bugs.

Rafael Maldonado wrote:
> Chris Fields wrote:
> >
> > My professor has several strains of E. coli and Salmonella in stab
> > cultures that are still viable after 20 years!  I think that you have to
> > be VERY careful with the phenotype.  A few of his were DOA due to
> > thymineless death (had a thyA mutation).  I guess his grad student at
> > the time had left out the thymidine; my prof was a bit pissed.
> PLasmids can be lost vith variable frequency in stab, even F primes...
> --
> Rafael Maldonado, Ph.D.
> Division of Genetics
> University of Alicante (Spain)

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