RNA resuspension problem

Stefi~ xxxgifalone at tin.it
Tue Jun 19 14:10:12 EST 2001


Melanie <mmhierl at yahoo.com> wrote in message
20010619150239.15314.qmail at ww02.jatek.com...
> I have in the past had no problems precipitationg and resuspending my RNA
in
> DEPC H20.  However, the last time I tried to do this using 1/10 volume
NaAc.........

Well, it could be dangerous for your RNA to be heatd at 65°C for much more.
Maybe you should consider to try another NaAc, or other EtOH. Maybe there's
something in those reagents. Be sure to use DEPC at 0,1% (v/v) and to
idrolize DEPC by autoclaving at 121°C for, at least, 30'. Let us know. Bye.
Stefano
gifalone at tin.it

http://web.tiscalinet.it/gifalone
ICQ 110419911





More information about the Methods mailing list