knck out strategy

lily king lily.king at cshs.org
Tue Jun 19 18:15:49 EST 2001


When I inject my  DNA  into the ES cell,it is better be linearlise DNA.
Does the DNA has to be included the whole NEO-plasmid?or it can be pop out
the rebination DNA,like this:
  sac11-TK-left arm-NEO-right arm-TK-sa11.
Thanks for helping me


<http://www.biowww.net/forum/read.php?f=1&i=2580&t=2580>




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