Plant PCR primers
Dr. Duncan Clark
news at hgmp.mrc.ac.uk
Wed Jun 20 03:14:17 EST 2001
In article <3B2F95D6.D89F0D11 at upei.ca>, the eminent Larry Hale at
University of Prince Edward Island wrote
> Can anyone tell me if they know of any PCR primers that are fairly
> reliable across most plant taxa (but more specifically Vaccinium)?
Universal plant primer sequences:
Primer 1: CGAAATCGGTAGACGCTACG
Primer 2: GGGGATAGAGGGACTTGAAC
1) 94°C - 1 minute
2) 94°C - 10 seconds
3) 55°C - 10 seconds Stages 2-4 repeated for 20-30cycles
4) 72°C - 30 seconds
>From Taberlet P. et al, Plant. Mol Biol. 17, 1105.
As shown on page 125 of Qiagen 2001 product guide.
They amplify the non-coding intergenic spacer between tRNAs.
Homogeneous Fluorescent Reporting Systems for Real-Time Quantitative PCR:
Optimisation, Probe Technology & Future Systems
4-5 September 2001
King Alfred's College, Winchester, UK
More information about the Methods