DNA won't dissolve into TE

R. Jayakumar jakku at mrna.tn.nic.in
Wed Jun 20 12:10:48 EST 2001

Strange.. we too use quiagen plasmids and other DNA prep kits and never had
this problem.  Do you dry the DNA too much.  What sort of material are you
using as the source

----- Original Message -----
From: "Marc Busch" <mgbusch at primate.ucdavis.edu>
To: <methods at hgmp.mrc.ac.uk>
Sent: Wednesday, June 20, 2001 10:27 PM
Subject: DNA won't dissolve into TE

> I have run into the problem (and so have others I know) that DNA from a
> qiagen prep (usually the endotoxin free kit) don't always go into
> suspension. White flakes (which seem to be DNA to me) just won't go into
> solution. I have tried pippeting up and down, vortexing, heat for a few
> hours at about 60 degrees, but it won't go into solution. I'm using TE
> Any ideas?
>       Marc Busch
>       Dept. Vir. and Immun.
>       CRPRC
>       Graduate Student
>       mgbusch at primate.ucdavis.edu


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