E. coli preservation

Chris Fields cjfields at jove.acs.unt.edu
Wed Jun 20 22:31:06 EST 2001


Yeah, that's why I always store plasmids and strains separately; any
strains that have a plasmid (such as an F' strain) are stored in the
-80.  

Rafael Maldonado wrote:
> 
> Chris Fields wrote:
> >
> > My professor has several strains of E. coli and Salmonella in stab
> > cultures that are still viable after 20 years!  I think that you have to
> > be VERY careful with the phenotype.  A few of his were DOA due to
> > thymineless death (had a thyA mutation).  I guess his grad student at
> > the time had left out the thymidine; my prof was a bit pissed.
> 
> PLasmids can be lost vith variable frequency in stab, even F primes...
> 
> --
> Rafael Maldonado, Ph.D.
> Division of Genetics
> University of Alicante (Spain)

-- 
C. J. Fields
Graduate Student, Dept. of Biological Sciences
The University of North Texas
Denton, TX 

email : cjfields at jove.acs.unt.edu
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