western for ZO-1

[Henk Veldman]

Siddhartha S Mitra schreef:

> can anyone give me the protocol for doing western blots for high
> molecular weight proteins (~220Kd) specifically ZO-1

See for instance:
Otter T, King SM, Witman GB (1987); A two step procedure for efficient
electrotransfer of both high-molecular-weight (>400,000) and
low-molecular-weight (<20,000) proteins. Anal Biochem 162:370-377

Henk
--
H. Veldman
Laboratory for Experimental Neurology (NMZ)
University Medical Center Utrecht (AZU)