Detecting sugars

kresten no-sp at m.org
Fri Jun 22 04:03:32 EST 2001


Mono- and oligosaccharides can be separated by high-pH anion exchange
chromatography and detected by pulsed amperometric detection. Dionex sells a
system for analysing this:

http://www.dionex.com/app/tree.taf?asset_id=7942

http://www.dionex.com/servletwl1/FileDownloader/slot114/9079/TN40.PDF

Good luck

Kresten

""Martin J. Cann"" <m.j.cann at durham.ac.uk> wrote in message
news:a05100301b7581ee723f1@[129.234.132.103]...
> I would like to look at various sugar levels e.g. G-1-P, R-1-P etc in
> bacteria grown under a variety of conditions.  Can anyone please
> point me in the direction of the best way to isolate the sugar pool
> and then to quantitate and identify, maybe mass spec but does someone
> know a better way, maybe HPLC based?
>
> Thanks in advance
>
> Martin Cann
> --
> Department of Biological Sciences
> Durham University
> South Road
> Durham DH1 3LE
> UNITED KINGDOM
>
> Phone: (+44) 191 374 3353
> Fax:  (+44) 191 374 2417
> e-mail: m.j.cann at durham.ac.uk
>
> ---





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