Is electrocompetent cell good for chemical transformation?

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Fri Jun 22 22:40:05 EST 2001


xinkunw at hotmail.com ("Xinkun Wang") wrote:
:Hi, there,
:
:I am wondering if electrocompetent cells are good for chemical 
:transformation. 

No. Absolutely not good.

:Would it give higher transformation efficiency? Or the same 
:efficiency as chemocompetent cells?

Lots, lots lower if you omit electric pulse.

"Electrocompetent" essentially means washed and 
resuspended in deionized water. 

"Chemically competent" means treated in a way that 
weakens cell wall and/or seriously disturbs plasma 
membrane (high Ca++, Li+, PEG, etc).

        - Dima




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