Is electrocompetent cell good for chemical transformation?
klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Fri Jun 22 22:40:05 EST 2001
xinkunw at hotmail.com ("Xinkun Wang") wrote:
:I am wondering if electrocompetent cells are good for chemical
No. Absolutely not good.
:Would it give higher transformation efficiency? Or the same
:efficiency as chemocompetent cells?
Lots, lots lower if you omit electric pulse.
"Electrocompetent" essentially means washed and
resuspended in deionized water.
"Chemically competent" means treated in a way that
weakens cell wall and/or seriously disturbs plasma
membrane (high Ca++, Li+, PEG, etc).
More information about the Methods