Detecting sugars

Dave Vadnais davevadnais at home.com
Mon Jun 25 20:48:35 EST 2001


You can also use a Waters Sugar-Pak column using isocratic elution on an
HPLC.  I used it to separate and quatitate sugars isolated from alfalfa and
it worked very well.

Dave Vadnais


"kresten" <no-sp at m.org> wrote in message
news:9gv1l4$t7k$1 at news.net.uni-c.dk...
> Mono- and oligosaccharides can be separated by high-pH anion exchange
> chromatography and detected by pulsed amperometric detection. Dionex sells
a
> system for analysing this:
>
> http://www.dionex.com/app/tree.taf?asset_id=7942
>
> http://www.dionex.com/servletwl1/FileDownloader/slot114/9079/TN40.PDF
>
> Good luck
>
> Kresten
>
> ""Martin J. Cann"" <m.j.cann at durham.ac.uk> wrote in message
> news:a05100301b7581ee723f1@[129.234.132.103]...
> > I would like to look at various sugar levels e.g. G-1-P, R-1-P etc in
> > bacteria grown under a variety of conditions.  Can anyone please
> > point me in the direction of the best way to isolate the sugar pool
> > and then to quantitate and identify, maybe mass spec but does someone
> > know a better way, maybe HPLC based?
> >
> > Thanks in advance
> >
> > Martin Cann
> > --
> > Department of Biological Sciences
> > Durham University
> > South Road
> > Durham DH1 3LE
> > UNITED KINGDOM
> >
> > Phone: (+44) 191 374 3353
> > Fax:  (+44) 191 374 2417
> > e-mail: m.j.cann at durham.ac.uk
> >
> > ---
>
>





More information about the Methods mailing list