Unknown Primer

Alexander Dobrovic alexander.dobrovic at adelaide.edu.au
Tue Jun 26 05:36:56 EST 2001


You first of all need to sequence your PCR product and then do databse
searches eg BLAST.

>Dear Colleagues,
>
>Following problem:
>
>We did RT-PCR for TGF-beta1 to test whether TGF-beta1 expression was
>downregulated on mRNA level by a certain drug.
>Due to a mistake, one primer contained a mmismatch at the 3' end. The
>mRNA we found was of different length than it should be (approx. 200Bp
>instead of 125) and could be downregulated by our drug (contrary to
>TGF-beta). We would be interested in finding out which protein does
>belong to this mRNA. Since we're not very well accoustomed with
>available databases, we would be very grateful for any hints how to do
>so.
>
>kind regards,
>
>T. Mohr



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