cloning of PCR products - errors?
Ian A. York
iayork at panix.com
Wed Jun 27 09:01:27 EST 2001
In article <3B39CD96.B8AB041 at bbm1.ucm.es>,
Sergio <sergioal at bbm1.ucm.es> wrote:
>Only 265x10-6 ??... damn... i must be doing something wrong. We use to get
>around 1x10-3 mutations when using Taq... almost five times the supposed error
I think the published error rates are for *optimized* PCR with the various
enzymes. Mixing Primer A and Primer B and throwing it in with template
isn't optimizing the reaction. I suspect that if you really fiddled with
the conditions you could get a much higher fidelity.
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
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