cloning of PCR products - errors?
sergioal at bbm1.ucm.es
Wed Jun 27 09:08:28 EST 2001
Roland Hubner wrote:
> In article <3B39CD96.B8AB041 at bbm1.ucm.es>, Sergio
> <sergioal at bbm1.ucm.es> wrote:
> > Only 265x10-6 ??... damn... i must be doing something wrong. We use to
> > get
> > around 1x10-3 mutations when using Taq... almost five times the supposed
> > error
> > rate.
> > Sergio
> cycle numbers?
MgCl2 2.5 mM
dNTPs 0.25 mM each
primers 0.5 uM each
95 (1') --- Tm-2 (2') -- 72 (1'/kb)
All reagents from Perkin Elmer
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