cloning of PCR products - errors?

Sergio sergioal at bbm1.ucm.es
Wed Jun 27 09:08:28 EST 2001



Roland Hubner wrote:

> In article <3B39CD96.B8AB041 at bbm1.ucm.es>, Sergio
> <sergioal at bbm1.ucm.es> wrote:
>
> > Only 265x10-6 ??... damn... i must be doing something wrong. We use to
> > get
> > around 1x10-3 mutations when using Taq... almost five times the supposed
> > error
> > rate.
> >
> > Sergio
>
>  cycle numbers?

25 cycles
MgCl2 2.5 mM
dNTPs 0.25 mM each
primers  0.5 uM each

95 (1') --- Tm-2 (2') -- 72 (1'/kb)

All reagents from Perkin Elmer

Sergio





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