"Pfx" DNA pol...
roland.hubner at ua.ac.be
Thu Jun 28 14:14:57 EST 2001
> Cycle sequencing is not PCR and, if I remember correctly, there is no
> patent on single primer extension.
What about this one?
United States Patent 4,962,020
Tabor, et. al. (Oct. 9, 1990)
A method for sequencing a strand of DNA, including the steps
of: providing the strand of DNA; annealing the strand with a
primer able to hybridize to the strand to give an annealed
mixture; incubating the mixture with a deoxyribonucleoside
triphosphate, a DNA polymerase, and a chain terminating
agent under conditions in which the polymerase causes the
primer to be elongated to form a series of DNA products
differing in length of the elongated primer, each DNA product
having a chain terminating agent at its elongated end; the
number of each DNA product being approximately the same for
substantially all DNA products differing in length from 1 to
In other words, even the Sanger centre will have to pay unless they
make sure some bands do not have same intensity !?!
ps. Whoah! KOD1 appears to have double processing speed compared with
Taq... really sort of yield polymerase...
More information about the Methods