cloning of PCR products - errors?

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Fri Jun 29 04:29:33 EST 2001


In article <3B3B62FD.F7CB2877 at Fackelmayer.de>,
 "Frank O. Fackelmayer" <Frank at Fackelmayer.de> wrote:

> First, these numbers are for optimized PCR reactions. MgCl2 and dNTP
> concentrations seem to be critical.

Absolutely.  Dropping the [dNTP] to a quarter of recommended values 
helps - it brings it into the Km of the enzyme after all.

r

-- 
Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
      -- 'There are no turtles anywhere' - Ponder Stibbons --




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