cloning of PCR products - errors?

Richard P. Grant rpg14 at
Fri Jun 29 04:29:33 EST 2001

In article <3B3B62FD.F7CB2877 at>,
 "Frank O. Fackelmayer" <Frank at> wrote:

> First, these numbers are for optimized PCR reactions. MgCl2 and dNTP
> concentrations seem to be critical.

Absolutely.  Dropping the [dNTP] to a quarter of recommended values 
helps - it brings it into the Km of the enzyme after all.


Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at'
      -- 'There are no turtles anywhere' - Ponder Stibbons --

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