cloning of PCR products - errors?
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Fri Jun 29 04:29:33 EST 2001
In article <3B3B62FD.F7CB2877 at Fackelmayer.de>,
"Frank O. Fackelmayer" <Frank at Fackelmayer.de> wrote:
> First, these numbers are for optimized PCR reactions. MgCl2 and dNTP
> concentrations seem to be critical.
Absolutely. Dropping the [dNTP] to a quarter of recommended values
helps - it brings it into the Km of the enzyme after all.
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
-- 'There are no turtles anywhere' - Ponder Stibbons --
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