Inverse PCR

Susanne Rohrer srohrer.nospam at immv.unizh.ch
Fri Mar 2 11:11:23 EST 2001



Rob Allen wrote:

>
>
> 4) Im using nested primers, which have a few b.p overlap- is this o.k?

Nested primers are a good idea, although you might get a product without
that if you're lucky, but if they overlap it increases your chance of
re-amplifying unspecific products in the second cycle.

Susanne







More information about the Methods mailing list