Anomalous cell growth on 96 well plates

Richard A. Hiipakka rhiipakk at
Fri Mar 2 15:53:29 EST 2001

Does anyone have an explanation for a gradient of cell growth in different
wells of a 96 well plate?  We have observed that wells nearer the periphery
of the plate have less cell growth when measured using assay based on MTT,
BUdr, or ATP.  Some people we have talked to even avoid using the outside
set of wells to minimize this phenomena and its affect on data.

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