E.coli lysis no enzyme damage

Emir Khatipov ekhatipo at NOSPAMmidway.uchicago.edu
Fri Mar 2 18:31:13 EST 2001


I sort of doubt myself that sonication inactivates DNAse I, but someone told
me and I follow, being lazy to check :)

"" J. Martinez-Irujo"" <jjmirujo at unav.es> wrote in message
news:3A9F6CF3.637EBE09 at unav.es...
> Emir Khatipov wrote:
>
> > I would freeze induced E.coli cell pellet at -80 to store,
> and when needed
> > thaw the pellet, add buffer with protease inhibitors,
> lysozyme and DNAse I
> > (~<10U/ml), freeze thaw the suspension couple of times.
> After
> > freeze/thawing, I would sonicate the lysates real quick (1
> x 15 sec; that
> > would kill DNAse I by the way), and spin down debris. Make
> sure no foam is
> > formed during sonication.
> >
> > Emir
>
> Do you know why sonication inactivates DNase I? Any
> reference?
>
> --
> Juan J. Martinez Irujo
> Universidad de Navarra
>
>
> ---







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