Please share experiences of using DEPC reated water?

Nick Theodorakis nicholas_theodorakis at urmc.rochester.ed
Fri Mar 2 21:50:40 EST 2001


In <45524439D771D311BF3E0090274F0456023B893E at wpg001ex6.gov.mb.ca>, "Hamel, Andre
(AGR wrote:
>
>  My question is, can anyone recommend any literature (BRL Focus, other
>journals) regarding the pros and cons of using DEPC treated water for
>molecular biology work?  And what is everyone elses experience with this
>apparent dilemma.
[...]

I stopped using it years ago, and my RNA has been fine. I just use fresh MilliQ
water or the equivalent right out of the tap. I figure if you don't need it, why
use it? If your water has RNAse in it, get better water; it probably has other
junk in it, too.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu






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