Anomalous cell growth on 96 well plates

Nick Theodorakis nicholas_theodorakis at urmc.rochester.ed
Fri Mar 2 21:52:05 EST 2001


In <rhiipakk-020301135329 at hiipakka-296c.bsd.uchicago.edu>, Richard A. Hiipakka
wrote:
>
>Does anyone have an explanation for a gradient of cell growth in different
>wells of a 96 well plate?  We have observed that wells nearer the periphery
>of the plate have less cell growth when measured using assay based on MTT,
>BUdr, or ATP.  Some people we have talked to even avoid using the outside
>set of wells to minimize this phenomena and its affect on data.

Evaporation? (then the medium becomes hypertonic)

Nick


-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu






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