Please share experiences of using DEPC reated water?
guerra at cmns.mnegri.it
Sun Mar 4 10:37:49 EST 2001
In my lab we use water for injecting (sterile, apirogenic) to work with RNA,
and we keep dedicated reagents and glassware inside a cabinet in the sterile
room - the glassware is not autoclaved but sterilized in the oven at 200°C
and something (I'll check the exact temperature) overnight - so far this is
working fine, with no need to use DEPC. Hope this is useful!
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