Searching a genome to find primer sequences that do not occur
rhubner at gins.uia.ac.be
Mon Mar 5 12:22:30 EST 2001
> Is there any way to search a small genome to find primer sequences that
> do not exist in that genome e.g. say anything from 15-22mers.
I know of 2 primer design programs that select most specific primers
according to the rarety of the 3'-nona/octamer sequence... Thusly, that
has certainly been done before (even when complete genome not
I am pretty sure that this was based on paper out in NAR years back...
(sorry, PubMed connection down right now here... title has very likely
word "octamer" in it)
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