silver stain negative results

R. John Lye rjl6n at virginia.edu
Mon Mar 5 16:50:22 EST 2001


lesley lancaster wrote:

> I am staining protein/polyacrylamide gels with bio-rad's silver stain plus.
> when i stain SDS gels, the bands look fine. when i stain native gels, my
> (snail) proteins do not show up, leaving only shadowy white bands in some
> lanes against the deepening background--

Are all the proteins doing this, or just the ones that you're interested
in?  There are some proteins that will stain with Coomassie, but
will not stain at all with silver.

Later,
John






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