polyclonal antibody production

Leen Leen.debolle at rega.kuleuven.ac.be
Tue Mar 6 04:58:16 EST 2001

I'ld like to immunize a rabbit to make polyclonal antibody for use in human
cell culture (lymphocytes). I therefore constructed a GST fusion protein
(Amersham technology), but the Factor Xa cut  that is required to obtain
pure protein doesn't seem to work well in my case. Can I use the fusion
protein for immunization or do the antibodies raised against the GST tag
interfere with human lymphocyte epitopes?



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