how to remove the NP40 from protein solutions

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Tue Mar 6 11:37:25 EST 2001


Krishnan,
did you considser salting out the protein and wash with high salt 
(i.e. same conc. of e.g. (NH4)2SO4) ? 

Wolfgang


> From:          RKrishnan at BIOCRYST.com ("Krishnan, Raman")
> Subject:       how to remove the NP40 from protein solutions
> Date:          6 Mar 2001 16:15:56 -0000
> Organization:  BIOSCI/MRC Human Genome Mapping Project Resource Centre
> X-To:          "'methods at net.bio.net'" <methods at net.bio.net>
> To:            methods at hgmp.mrc.ac.uk

> i wish to crystallize a protein which has np40 in it?
> how do i get rid of it....
> iam not equiped with FPLC....
> only centrifuges...
> 
> Raman Krishnan
> Research Scientist
> Biocryst
> 2190, Parkway Lake Drive
> Birmingham
> AL 35244
> off. 205-403-4677
> home. 205-733-0981
> EMail: RKrishnan at BIOCRYST.com
> FAX: +1 205 444-4640
> http://www.biocryst.com/
> 
> 
> Have a nice day!!
> 
> 
> ---
> 
> 
> 
> 

-----
Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
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Taipei 115
Taiwan R.O.C.
Tel +886-2-2798-9152
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Mobile +886-925-136893
wolfsc at ibms.sinica.edu.tw
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