Anti-His6 vs Ni-labeled HRP

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Tue Mar 6 11:54:48 EST 2001


lhom at OCF.Berkeley.EDU (Louis Hom) wrote:
:Does anyone have comments on the relative merits of using Anti-His6 Ab 
:versus Ni-labeled HRP to detect His-tagged proteins?

I seem to remember that anti-6his Ab, even though it
is really low affinity, is still more sensitive. 
No suprise here since another layer of amplification
is present.

        - Dima






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