how to remove the NP40 from protein solutions

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Tue Mar 6 11:56:23 EST 2001


RKrishnan at BIOCRYST.com ("Krishnan, Raman") wrote:
:i wish to crystallize a protein which has np40 in it?
:how do i get rid of it....
:iam not equiped with FPLC....
:only centrifuges...

The best way:

Bind to any column the protein binds tightly to,
wash extensively, elute. Hydroxylapatite is
generally a good thing to start - binds almost
all proteins in the absence of phosphate. 
You don't need FPLC to do this, not even LC -
simple gravity column will work. 

        - Dima






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