Real Time PCR Controls...
Dr. Duncan Clark
d_clark at nospam.demon.co.uk
Wed Mar 7 04:20:34 EST 2001
In article <3aa59f61.443734 at news-east.usenetserver.com>, the eminent
Rick Davis at WebUseNet Corp http://www.usenetserver.com - Home of the
fastest NNTP servers on the Net. wrote
>We're using ABI's 5700 Sequence Detection system for relative gene
>expression quantitation in human keratinocytes.. Our ABI guru told us
>that 18S Ribosomal RNA is the best endogenous control to use in these
>types of measurements. Unfortunately ABI will not disclose the primer
>pairs they use in the TaqMan 18S kits that they sell. Is anyone out
>there that is doing real time PCR applications using 18S as a control
>willing to share their primer pair sequences with me?
Try asking the gurus on the 7700 listserv.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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