Real Time PCR Controls...

[Dr. Duncan Clark]

In article <3aa59f61.443734 at news-east.usenetserver.com>, the eminent
Rick Davis at WebUseNet Corp  http://www.usenetserver.com - Home of the
fastest NNTP servers on the Net. wrote
>We're using ABI's 5700 Sequence Detection system for relative gene
>expression quantitation in human keratinocytes.. Our ABI guru told us
>that 18S Ribosomal RNA is the best endogenous control to use in these
>types of measurements. Unfortunately ABI will not disclose the primer
>pairs they use in the TaqMan 18S kits that they sell. Is anyone out
>there that is doing real time PCR applications using 18S as a control
>willing to share their primer pair sequences with me? 
>
>Thanks,
>Rick
>

Try asking the gurus on the 7700 listserv.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk