how to remove the NP40 from protein solutions
mw132 at mole.bio.cam.ac.uk
Wed Mar 7 08:13:45 EST 2001
I am trying a similar thing - crystallization of a protein
which I have dissolved in 0.01% Triton X100. My advisor says that this
amount of deterent may not be a problem. Could the same apply to you?
Anyway, Calbiochem sell beads for absorbing detergents. They may help.
On 6 Mar 2001, Krishnan, Raman wrote:
> i wish to crystallize a protein which has np40 in it?
> how do i get rid of it....
> iam not equiped with FPLC....
> only centrifuges...
> Raman Krishnan
> Research Scientist
> 2190, Parkway Lake Drive
> AL 35244
> off. 205-403-4677
> home. 205-733-0981
> EMail: RKrishnan at BIOCRYST.com
> FAX: +1 205 444-4640
> Have a nice day!!
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