how to remove the NP40 from protein solutions

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Mar 7 08:13:45 EST 2001


Dear Krishnan,
             I am trying a similar thing - crystallization of a protein
which I have dissolved in 0.01% Triton X100.  My advisor says that this
amount of deterent may not be a problem.  Could the same apply to you?

Anyway, Calbiochem sell beads for absorbing detergents.  They may help.

Regards, Mike.

On 6 Mar 2001, Krishnan, Raman wrote:

> i wish to crystallize a protein which has np40 in it?
> how do i get rid of it....
> iam not equiped with FPLC....
> only centrifuges...
> 
> Raman Krishnan
> Research Scientist
> Biocryst
> 2190, Parkway Lake Drive
> Birmingham
> AL 35244
> off. 205-403-4677
> home. 205-733-0981
> EMail: RKrishnan at BIOCRYST.com
> FAX: +1 205 444-4640
> http://www.biocryst.com/
> 
> 
> Have a nice day!!
> 
> 
> ---
> 
> 






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