alkaline phosphatase substrate solution

Andy Puttmann puttand at bvu.edu
Wed Mar 7 19:36:31 EST 2001


I am preparing to do an ELISA and all the information that I've found
says to use a substrate/diethanolamine solution. The only different data
that I found said to use a substrate/PBS solution. I have PBS on hand,
but I do not have any diethanolamine. I would prefer to use the PBS, but
I don't have any of the specifics or mixing ratios. If anyone could help
me out it would be greatly appreciated.

Thanks,
Andy


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