alkaline phosphatase substrate solution

NJ njuni at
Wed Mar 7 23:56:43 EST 2001

Substrate/diethanolamine solution should be for alkaline phosphatase
reaction. It is my understanding that PBS should be avoided for alkaline
phosphatases since phosphate inhibits their activity. A commonly-used
alternative to diethanolamine for this purpose is Tris (pH9.5), e.g.100 mM
NaCl/ 5 mM MgCl2/ 100 mM Tris (pH 9.5). PSB is ok for other enzymes such as

in article 3AA6D3B3.F49686B6 at, Andy Puttmann at puttand at wrote
on 3/8/01 9:36 AM:

> I am preparing to do an ELISA and all the information that I've found
> says to use a substrate/diethanolamine solution. The only different data
> that I found said to use a substrate/PBS solution. I have PBS on hand,
> but I do not have any diethanolamine. I would prefer to use the PBS, but
> I don't have any of the specifics or mixing ratios. If anyone could help
> me out it would be greatly appreciated.
> Thanks,
> Andy
> ---

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