isoamyl alcohol

Arnoud van Vliet avvliet at knoware.nl
Thu Mar 8 06:11:02 EST 2001


> i know that phenol/chloroform/isoamyl alcohol should solubilize unwanted
> proteins and lipids, but does anyone know if it will also take out SDS
> from my aqueous DNA?
> any info would be appreciated.

Ronald,

PCI extraction will not take out SDS, as far as I know. To get rid of SDS,
perform a sodium acetate/ethanol precipitation, or alternatively use a
silica-based spin column sold by numerous companies like Qiagen, Promega etc
etc.

hope this helps
Arnoud







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