isoamyl alcohol

[Arnoud van Vliet]

> i know that phenol/chloroform/isoamyl alcohol should solubilize unwanted
> proteins and lipids, but does anyone know if it will also take out SDS
> from my aqueous DNA?
> any info would be appreciated.

Ronald,

PCI extraction will not take out SDS, as far as I know. To get rid of SDS,
perform a sodium acetate/ethanol precipitation, or alternatively use a
silica-based spin column sold by numerous companies like Qiagen, Promega etc
etc.

hope this helps
Arnoud