pGEM-T easy vector gives extra band

Dr. Duncan Clark d_clark at
Thu Mar 8 08:58:47 EST 2001

In article <Pine.LNX.4.21.0103072328550.11716-100000 at biochem.iisc.ernet.
in>, the eminent SURESH KUMAR K.G. at BIOSCI/MRC Human Genome Mapping
Project Resource Centre wrote
>Prepping yourself means, you transform strains like JM109/XL1-Blue
>with your vector (pGEMT), pick single colonies, do mini/midi/maxi preps,
>You will have more than enough vector for a life time...
>Hope it is clear..

That will not work. pGEMT is a linear Blunt RE digested plasmid. It is
then given a single base overhang either by TdT or Taq plus ddTTP etc.
You cannot generate that vector juts by transformation etc.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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