pGEM-T easy vector gives extra band

Dr. Duncan Clark d_clark at nospam.demon.co.uk
Thu Mar 8 08:58:47 EST 2001


In article <Pine.LNX.4.21.0103072328550.11716-100000 at biochem.iisc.ernet.
in>, the eminent SURESH KUMAR K.G. at BIOSCI/MRC Human Genome Mapping
Project Resource Centre wrote
>Prepping yourself means, you transform strains like JM109/XL1-Blue
>with your vector (pGEMT), pick single colonies, do mini/midi/maxi preps,
>You will have more than enough vector for a life time...
>Hope it is clear..

That will not work. pGEMT is a linear Blunt RE digested plasmid. It is
then given a single base overhang either by TdT or Taq plus ddTTP etc.
You cannot generate that vector juts by transformation etc.

Duncan 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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