pGEM-T easy vector gives extra band
sergioal at bbm1.ucm.es
Thu Mar 8 09:13:58 EST 2001
"R. Jayakumar" wrote:
> [...] T-tailing it with
> DNA polymerase and an excess of TTP, which I can very well do in some other
> vector too. it is this protocol which I wanted.
Try using the terminal transferase from any vendor, adding dTTP to a blunt end.
I don't have the protocol right here, but i think there is a brief one in
Sambrook, and in some catalogs.
Hope this helps
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