pGEM-T easy vector gives extra band

Sergio sergioal at bbm1.ucm.es
Thu Mar 8 09:13:58 EST 2001



"R. Jayakumar" wrote:

> [...] T-tailing it with
> DNA polymerase and an excess of TTP, which I can very well do in some other
> vector too.    it is this protocol which I wanted.

Try using the terminal transferase from any vendor, adding dTTP to a blunt end.
I don't have the protocol right here, but i think there is a brief one in
Sambrook, and in some catalogs.

Hope this helps

Sergio






More information about the Methods mailing list