alkaline phosphatase substrate solution
johnstoj at acs.ucalgary.ca
Thu Mar 8 15:36:41 EST 2001
Let's clear this up. The alkaline phosphatase reaction uses BCIP
(bromo-chloro-indolyl-phosphate) and NBT (nitro-blue tetrazolium). The BCIP is
soluble only in diethanolamine or DMSO if you have the toluidine salt. If
however you are using the disodium salt then it is soluble in aqueous solutions
(PBS, TBS etc). NBT is soluble in DMSO.
The staining solution (50mL) is 0.2M Tris-HCl pH8.8, 0.8mM MgCl2, 5mg NBT and
There are probably other solutions out there, but this is the one I use.
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