th-system: interaction strength expression level dependent?

Frederik Börnke ricky_boernke at
Fri Mar 9 03:05:29 EST 2001

Dear all,

my protein of interest interacts with a target protein in an
isoform-specific manor, as determined by quantitative b-gal assays.
Binding of one of the isoforms is rather strong, another binds not
at all and some more are intermediate. When I tried to publish that
the reviewer wanted me to assure equal level of expression of all
isoforms by western-blotting. I agree that I should check expression
of the isoform which does not interact at all, but anyway, is 
interaction strength of two-hybrid proteins linked to expression
level of one of the proteins? In particular, I am using truncated
versions of the adh promoter, making western detection of hybrid
proteins a bit difficult since expression is supposed to be below
the detection level.
Any input appreciated.



Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515

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