pGEM-T easy vector gives extra band
Dr. Duncan Clark
d_clark at nospam.demon.co.uk
Fri Mar 9 04:17:13 EST 2001
In article <email@example.com>, the eminent Michael
L. Sullivan at BIOSCI/MRC Human Genome Mapping Project Resource Centre
>I've used this a sseveral times. I found that I got rather a lot of
>background, BUT, if you use a vector with color selection, it is easy to
>pick clones with inserts.
Tail, ligate and gel purify the linear band. Use that. That should
remove background due to non-tailed, still blunt vector.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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