pGEM-T easy vector gives extra band

Dr. Duncan Clark d_clark at nospam.demon.co.uk
Fri Mar 9 04:17:13 EST 2001


In article <v04011700b6cd4fc6879a@[144.92.64.179]>, the eminent Michael
L. Sullivan at BIOSCI/MRC Human Genome Mapping Project Resource Centre
wrote
>I've used this a sseveral times.  I found that I got rather a lot of
>background, BUT, if you use a vector with color selection, it is easy to
>pick clones with inserts.

Tail, ligate and gel purify the linear band. Use that. That should
remove background due to non-tailed, still blunt vector.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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