pGEM-T easy vector gives extra band

Dr. Duncan Clark d_clark at
Fri Mar 9 04:19:06 EST 2001

In article <3AA793A5.74B141AB at>, the eminent Sergio at UCM
>Try using the terminal transferase from any vendor, adding dTTP to a blunt end.
>I don't have the protocol right here, but i think there is a brief one in
>Sambrook, and in some catalogs.

Won't just using TTP generate a long multi-T tail. Should one use ddTTP?
I need to check the original references but I think it was dTTP and Taq
or ddTTP and TdT.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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