pGEM-T easy vector gives extra band

Dr. Duncan Clark d_clark at nospam.demon.co.uk
Fri Mar 9 04:19:06 EST 2001


In article <3AA793A5.74B141AB at bbm1.ucm.es>, the eminent Sergio at UCM
wrote
>Try using the terminal transferase from any vendor, adding dTTP to a blunt end.
>I don't have the protocol right here, but i think there is a brief one in
>Sambrook, and in some catalogs.

Won't just using TTP generate a long multi-T tail. Should one use ddTTP?
I need to check the original references but I think it was dTTP and Taq
or ddTTP and TdT.

Duncan 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
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http://www.dnamp.com
http://www.genesys.demon.co.uk






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