I have a large genomic fragment of DNA,which i have digested with ECO-RI for testing on southerns,however after digestion of between 1-5ug i find it hard to resuspend the precipated pellet,also the bands on an 0.8% agarose gel are very smeary.can anyone help out? Ed. <http://www.biowww.net/forum/read.php?f=1&i=1155&t=1155>