molecular biology

Nick Theodorakis nicholas_theodorakis at urmc.rochester.ed
Fri Mar 9 15:41:34 EST 2001


In <20010309172239.26694.qmail at ww02.jatek.com>, ed stubs wrote:
>
>I have a large genomic fragment of DNA,which i have digested with ECO-RI for
>testing on southerns,however after digestion of between 1-5ug i find it hard
>to resuspend the precipated pellet,also the bands on an 0.8% agarose gel are
>very smeary.can anyone help out?

Did you perhaps overdry it, e.g., with a speed-vac? Overdry DNA is hard to
resuspend.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu






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