Methanol Fixing of Proteins

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Fri Mar 9 19:32:24 EST 2001


jhaines at uoguelph.ca (Jeffery Haines) wrote:
:Just wondering if anyone knows the specific mechanism of why methanol 
:when included with Coomassie Stain "fixes" proteins to the PAGE gel?

Denaturation and "non-specific" interaction with the gel.
That's why small proteins are harder to fix. 

        - Dima







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