molecular biology

[Dr. Hiranya S. Roychowdhury]

Resuspension:

Do not dey the DNA pellet completely. Better yet, do not dry at all before
adding the resuspension buffer (TE8) or water. If DNA is good, it goes into
solution very quickly when slightly wet. Following resuspension, speed vac
the solution for a minute to get rid of residual EtOH.

Smear:

You should expect a smear when you digest genomic DNA, with very faint
ladder showing through. 


At 05:22 PM 3/9/01 -0000, ed stubs wrote:
>I have a large genomic fragment of DNA,which i have digested with ECO-RI for
>testing on southerns,however after digestion of between 1-5ug i find it hard
>to resuspend the precipated pellet,also the bands on an 0.8% agarose gel are
>very smeary.can anyone help out?
>Ed.
>
>
><http://www.biowww.net/forum/read.php?f=1&i=1155&t=1155>
>
>
>
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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